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The left ventricle was fixed with formalin at pressure equal to 13 cm water column, and the postmortem LV volumes were measured by filling the cavity with water and weighing, repeated three times and averaged

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Although the matched estimates remained directionally consistent with the regression findings, the loss of statistical significance after balancing observed confounders suggests that unmeasured confounding particularly by body mass index and health motivation likely explains a substantial share of the associations detected in conventionally adjusted models